Device

Part:BBa_K771000:Design

Designed by: GUO Huaqing;SUO Yang;WU Yuqi   Group: iGEM12_SJTU-BioX-Shanghai   (2012-09-25)


Membrane Anchor 0:ssDsbA-Bla-LGT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1747
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 794
    Illegal BsaI site found at 1368


Design Notes

For Membrane Localization


Source

ssDsbA is from E.coli genome; β-lactamase is from pET-Duet1; Lgt is from E.coli genome

References